目的 考察不同灭菌方法对白热斯灭菌效果及其活性成分含量的影响,选择最适宜的灭菌方法。方法 分别采用热压灭菌、流通蒸气灭菌、干热灭菌、60Co辐射灭菌4种不同的灭菌方法对白热斯药材粉末进行灭菌处理,采用HPLC测定白热斯中胡椒碱、异绿原酸A、异绿原酸C、槲皮素4种指标性成分的含量。比较不同灭菌方法对白热斯中4种指标性成分含量及微生物限度的影响。结果 4种灭菌方法所检测的细菌总数、霉菌、酵母菌和大肠埃希菌均符合《中国药典》的要求。60Co辐照灭菌,辐射剂量为4 kGy时,4种指标性成分总含量高于其他灭菌方法,损失率为1.21%。结论 白热斯灭菌方法选用60Co辐射灭菌技术,工艺简单,干燥时间短,且能有效保留各指标成分。
Abstract
OBJECTIVE To compare the contents of the index components in Baras treated by different sterilization methods for choosing the best sterilization method.METHODS The mixed medicinal powder of Baras were treated by autoclave sterilization, circulating steam sterilization, dry heat sterilization and 60Co radiation sterilization, respectively. The contents of iso-chlorogenic acid A, iso-chlorogenic acid C, quercetin and piperine were determined by HPLC. The influence on the contents of the index components and microbe limitation were analyzed.RESULTS The total number of bacteria and the counts of mold, yeast count and Escherichia coli in the samples treated by the four sterilization methods were all in compliance with the requirements of Chinese Pharmacopoeia. The total content of four kinds of index components in the samples treated by 60Co radiation sterilization method at dose of 4 kGy was slightly higher than that of others, with a loss rate of 1.21%.CONCLUSION The 60Co radiation sterilization method is suitable for Baras drying with simple process, short drying time, and the ability to effectively retain all index components.
关键词
白热斯 /
灭菌方法 /
胡椒碱 /
异绿原酸A /
异绿原酸C /
槲皮素 /
60Co辐射灭菌
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Key words
Baras /
sterilization method /
piperine /
iso-chlorogenic acid A /
iso-chlorogenic acid C /
quercetin /
60Co radiation sterilization method
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中图分类号：
R917
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参考文献
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脚注
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基金
国家“重大新药创制”科技重大专项课题资助(2017ZX09301060-005);新疆维吾尔自治区重点研发计划项目资助(2016B03038-3-3)
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